Guarana (Paullinia cupana) Extract Protects Caenorhabditis elegans Models for Alzheimer Disease and Huntington Disease through Activation of Antioxidant and Protein Degradation Pathways

Figure 3

Effect of guarana hydroalcoholic extract (GHE) treatment on C. elegans lifespan and stress resistance. (a) Survival curves of wild-type (N2) animals under standard laboratory conditions. Worms were treated with either 10 or 50 mg/mL GHE beginning at L1. Survival was verified every day at 25°C and compared to respective controls, log-rank (Mantel-Cox) test. (b) Survival curves of wild-type (N2) animals under oxidative stress conditions. Worms were treated with either 10 or 50 mg/mL GHE from L1 until L4 and transferred to plates with 10 mM TBPH. Survival was verified every 3 hours at 20°C. No significant differences were seen between groups, determined by a log-rank (Mantel-Cox) test. (c) Survival curves of wild-type (N2) animals under heat stress. Worms were treated with either 10 or 50 mg/mL GHE from L1 until 5 days of age and incubated at 35°C. The survival was verified 3 h at 35°C. compared to respective controls, log-rank (Mantel-Cox) test.

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