Pharmaceutical Induction of PGC-1α Promotes Retinal Pigment Epithelial Cell Metabolism and Protects against Oxidative Damage

Table 1

Primer sequences used for gene expression experiments.

Gene symbol	Gene name	Forward sequence (5–3)	Reverse sequence (5–3)
CRYAB	Crystallin alpha B	GTTCTTCGGAGAGCACCTGTT	GAGAGTGCAGTGTCAAACCAG
PPIH	Peptidylprolyl isomerase H	CCCCAACAATAAGCCCAAG	CACCACCAAGAAGAAGGGAA
HPRT1	Hypoxanthine phosphoribosyltransferase 1	CCTGGCGTCGTGATTAGTGAT	AGACGTTCAGTCCTGTCCATAA
β-Actin	Beta-actin	CTGTCTGGCGGCACCACCAT	GCAACTAAGTCATAGTCCGC
PGC-1α	Peroxisome proliferator-activated receptor gamma coactivator 1 alpha	GTCACCACCCAAATCCTTAT	ATCTACTGCCTGGAGACCTT
PGC-1β	Peroxisome proliferator-activated receptor gamma coactivator 1 beta	CCACATCCTACCCAACATCAAG	CACAAGGCCGTTGACTTTTAGA
ESRRA	Estrogen-related receptor alpha	TATGGTGTGGCATCCTGTG	GTCTCCGCTTGGTGATCTC
FOXO1	Forkhead box O1	AAGAGCGTGCCCTACTTCAA	GCACACGAATGAACTTGCTG
FOXO3	Forkhead box O3	CTTCAAGGATAAGGGCGACA	AGTTCCCTCATTCTGGACCC
NRF1	Nuclear respiratory factor 1	GCTGATGAAGACTCGCCTTCT	TACATGAGGCCGTTTCCGTTT
NRF2	Nuclear factor, erythroid 2 like 2	TCCAGTCAGAAACCAGTGGAT	GAATGTCTGCGCCAAAAGCTG
PPARα	Peroxisome proliferator-activated receptor alpha	ATCGAATGTAGAATCTGCGGG	TCGCACTTGTCATACACCAG
ATP5O	ATP synthase subunit O, mitochondrial	TTTGAATCCCTATGTGAAGCGTT	CCTTGGGTATTGCTTAATCGACC
COX4I1	Cytochrome c oxidase subunit 4 isoform 1	GCACTGAAGGAGAAGGAGAAG	AACCGTCTTCCACTCGTTC
COX5B	Cytochrome c oxidase subunit 5B	GGAAGACCCTAATTTAGTCCCCT	CCAGCTTGTAATGGGCTCCAC
NDUFB5	NADH-ubiquinone oxidoreductase subunit B5	CACTCGCCTCGGATTTGG	CGCCTGTCATAGAATCTAGAAGG
CAT	Catalase	ACTTTGAGGTCACACATGACATT	CTGAACCCGATTCTCCAGCA
GPX1	Glutathione peroxidase 1	CCAGTCGGTGTATGCCTTCTC	GAGGGACGCCACATTCTCG
TXN2	Thioredoxin 2	TGATGACCACACAGACCTCG	ATCCTTGATGCCCACAAACT
SOD1	Superoxide dismutase 1	AGGGCATCATCAATTTCGAGC	GCCCACCGTGTTTTCTGGA
SOD2	Superoxide dismutase 2	CAGACCTGCCTTACGACTATGG	CGTTCAGGTTGTTCACGTAGG
PRDX3	Peroxiredoxin 3	GATTTCCCGAGACTACGGTG	GACGCTCAAATGCTTGATGA
PRDX5	Peroxiredoxin 5	AGTGAAGGAGAGTGGGCGTC	TTCAAACACCTCCACTGCTG
MFN1	Mitofusin 1	TGCCCTTCACATGGACAAAG	CTCTGTAGTGACATCTGTGCC
FIS1	Fission 1	TGACATCCGTAAAGGCATCG	CTTCTCGTATTCCTTGAGCCG
TFAM	Transcription factor A, mitochondrial	CCATATTTAAAGCTCAGAACCCAG	CTCCGCCCTATAAGCATCTTG
POLG	DNA polymerase gamma	GAAGGACATTCGTGAGAACTTCC	GTGGGGACACCTCTCCAAG
MITF	Microphthalmia-associated transcription factor	AGCCATGCAGTCCGAAT	ACTGCTGCTCTTCAGCG
BEST1	Bestrophin 1	GAATTTGCAGGTGTCCCTGT	ATCCTCCTCGTCCTCCTGAT
RLBP1	Retinaldehyde-binding protein 1	CACGCTGCCCAAGTATGATG	CCAGGACAGTTGAGGAGAGG