Research Article
In Vivo Evaluation of the Antioxidant Activity and Protective Action of the Seaweed Gracilaria birdiae
Figure 7
Effect of GB extracts on 3T3-L1 cells. (a) Cytotoxicity of GB extracts. Cytotoxicity of extracts was determined using the MTT assay. The cells were seeded at a density of 1 × 105 cells/mL in 96-well plates and treated with GB extracts for 24, 48, and 72 h. The optical density of cells not exposed to extracts was considered as 100% of relative MTT reduction. (b) GB extracts inhibit intracellular lipid accumulation in 3T3-L1 adipocytes. 3T3-L1 cells stained with Oil Red O after 15 days of treatment with the extracts. Several drops of lipids can be observed within the cytoplasm of cells that differentiated into adipocytes. Bar = 20 μm. (c) Overview of wells following the elution of Oil Red O from within the cells. Note that the cells treated with AE are stained with a lighter color. (d) Stained oil droplets were dissolved with isopropanol and evaluated by spectrophotometric analysis at 510 nm. The optical density of cells treated only with MDI was considered to be 100% relative lipid content. Values are expressed as mean ± standard deviation. ; .
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