Antioxidant and Antisenescence Effects of Bergamot Juice

Table 1

Nucleotide sequences, annealing temperature, and product size of the primers utilized in real-time RT-PCR experiments.

		Primer nucleotide sequences	Annealing temp (°C)	Product size (base pairs)
SIRT1	For. 5	ATGACGCTGTGGCAGATTGTT	66.8	202
	Rev. 5	CCGCAAGGCGAGCATAGAT	67.4
NRF2	For. 5	GGACATGGAGCAAGTTTGGC	66.8	165
	Rev. 5	TCCAGCGAGGAGATCGATGA	68.4
FOXO3	For. 5	AGTGGATGGTGCGCTGTGT	67.1	100
	Rev. 5	CTGTGCAGGGACAGGTTGT	64.0
HO-1	For. 5	ATACCCGCTACCTGGGTGAC	64.9	200
	Rev. 5	TGTCACCCTGTGCTTTGACCT	67.0
NQO1	For. 5	TTCTGTGGCTTCCAGGTCTT	63.8	130
	Rev. 5	AGGCTGCTTGGAGCAAAATA	63.7
GAPDH	For. 5	ATGTGTCCGTCGTGGATCTGAC	68.5	132
	Rev. 5	AGACAACCTGGTCCTCAGTGTAG	63.4