Exogenous Hydrogen Sulfide Supplement Attenuates Isoproterenol-Induced Myocardial Hypertrophy in a Sirtuin 3-Dependent Manner

Figure 3

Effect of NaHS on plasma H₂S level and myocardial H₂S production in WT mice and SIRT3 KO mice after ISO administration. Male 129S1/SvImJ (WT) and SIRT3 knockout (KO) mice at 10 weeks of age were administrated with NaHS (50 μmol/kg/d) or normal saline (NS) once daily. After 2 weeks, the mice were given isoproterenol (ISO, 60 mg/kg) by intraperitoneal injection followed by NaHS or NS administration once daily for another 2 weeks. (a–b) Plasma H₂S level and myocardial H₂S production were measured with H₂S specific microelectrodes connected to the free radical analyzer. (c–e) CSE, CBS, and MPST mRNA expressions were measured by quantitative real-time PCR. 18S was serviced as a house-keep gene. Plots represent the mean ± SEM; . Statistical significance: , compared with WT + NS; compared with WT + ISO; , compared with SIRT3 KO + NS; , compared with SIRT3 KO + ISO.

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