[Retracted] 5-Aminolevulinic Acid-Based Photodynamic Therapy Pretreatment Mitigates Ultraviolet A-Induced Oxidative Photodamage

Figure 2

UVA-induced oxidative damage was reduced with ALA-PDT pretreatment. (a, b) Cells were treated with a sublethal dose of ALA-PDT, then these cells were irradiated with 10 J/cm² of UVA light. Cells were incubated with 10 μmmol/L DCFH-DA or 5 μmmol/L Dihydroethidium for 20 min, after which ROS and O₂⁻ production was visualized by fluorescence microscopy. Intracellular ROS and O₂⁻ species exhibited green and red fluorescence under a fluorescence microscope; the fluorescence intensity was determined by a fluorescence microplate reader. Data were expressed as mean ± SD, for each group, .

(a)

(b)