Gankyrin Drives Malignant Transformation of Gastric Cancer and Alleviates Oxidative Stress via mTORC1 Activation

Figure 1

Gankyrin contributes to the early malignant transformation of gastric cancer. (a) Immunohistochemistry (IHC) staining of gankyrin in noncancerous and precancerous gastric tissue sections. Representative images of stained nonatrophic gastritis (NAG), chronic atrophic gastritis (CAG), CAG with intestinal metaplasia (IM), and CAG with dysplasia (dys) gastric tissue sections (original magnification, 100x or 400x) are shown. (b) Scatter plot showing gankyrin staining level in individual noncancerous and precancerous gastric tissue sections, 30 cases for each group. (c) IHC staining of human gastric cancer tissue and paired noncancerous tissues. Representative images of the stained tumor and paired noncancerous tissue are shown with strong, moderate, and weak positivity for gankyrin expression. (d) Box plot graph showing the statistical analysis of gankyrin expression in 77 gastric cancers and paired noncancerous tissues. (e) Gankyrin mRNA expression was significantly upregulated in gastric cancer tissues as compared to normal gastric tissues based on microarray data of GSE26942 (). (f) Kaplan-Meier survival analysis of gastric cancer cases divided into two groups by the median value (H-score = 145) for gankyrin staining. The value was calculated by the log-rank test.

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