Resveratrol-Induced Downregulation of NAF-1 Enhances the Sensitivity of Pancreatic Cancer Cells to Gemcitabine via the ROS/Nrf2 Signaling Pathways

Figure 4

Resveratrol inhibits the expression of NAF-1 in pancreatic cancer cells via activating the Nrf2 pathways. (a–d) The effects of gradient concentrations of resveratrol on the protein expression levels of NAF-1 and Nrf2 were examined by a Western blot analysis using α-tubulin as an internal loading control; quantified histograms of the western blots were shown alongside. (e, f) Immunofluorescence staining of NAF-1 in Panc-1 and Mia paca-2 cells was performed to show the effects of resveratrol on the protein expression of NAF-1 after treatment with resveratrol (50 μM and 100 μM) for 24 h. (g, h) Immunofluorescence staining of Nrf2 showed the expression level and translocation of Nrf2 under the resveratrol stimulation in Panc-1 and Mia paca-2 cells (magnification, 400x; scale bar, 20 μm). (i–k) After transfection with siNrf2 or siControl for 48 h, the total proteins in the Panc-1 and Mia paca-2 cells were collected, and the protein expression levels of NAF-1 and Nrf2 were examined by a Western blot analysis using α-tubulin as an internal loading control and quantified histograms of the western blots were shown alongside. Column: mean; bar: SD. compared with NAF-1 control group. compared with control group.

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