Perillaldehyde Inhibits AHR Signaling and Activates NRF2 Antioxidant Pathway in Human Keratinocytes

Figure 1

(a) HaCaT keratinocytes were treated with BaP in the presence or absence of PAH (1, 10, 100, or 1000 μM), and CYP1A1 expression was measured by quantitative RT-PCR. . . (b) Subcellular localization of AHR was visualized by immunofluorescence. Cells were stained with anti-AHR antibody (green) and 4,6-diamidino-2-phenylindole (blue). BaP induced cytoplasmic to nuclear translocation of AHR, which was blocked by PAH. (c) The proportion of keratinocytes in which AHR was predominantly stained in the nucleus was calculated. .

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