Research Article
Perillaldehyde Inhibits AHR Signaling and Activates NRF2 Antioxidant Pathway in Human Keratinocytes
(a) |
| | Reagent |
| | Perillaldehyde (PAH) | Tokyo Chemical Industry (Tokyo, Japan) | | Dulbecco’s modified Eagle’s medium | Sigma-Aldrich (St. Louis, MO, USA) | | Benzo(a)pyrene (BaP) | | Dimethyl sulfoxide (DMSO) | | Ethanol | Wako Laboratory Chemicals (Osaka, Japan) | | 0.05% (w/v) trypsin-0.53 mM EDTA-4Na solution |
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(b) |
| | Antibody |
| | Anti-AHR rabbit polyclonal antibody (H-211) | Santa Cruz Biotechnology (Dallas, TX, USA) | | Anti-NRF2 rabbit polyclonal antibody (H-300) | | UtraCruzTM mounting medium containing 4,6-diamidino-2-phenylindole | | Horseradish peroxidase-conjugated green-fluorescent Alexa Flour® 488 | Molecular Probes (Eugene, OR, USA) | | 5-(and −6)-Carboxy-2,7-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) |
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(c) |
| | Kit |
| | RNeasy Mini kit | Qiagen (Hilden, Germany) | | PrimeScriptTM RT reagent | Takara Bio (Kusatsu, Japan) | | SYBR® Premix Ex TaqTM II | | Quantikine human CCL2/MCP1 ELISA kit | R&D Systems (Minneapolis, MN, USA) | | Quantiline human IL-1β ELISA kit | | CCK-8 assay kit | Dojindo Laboratories (Kumamoto, Japan) |
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(d) |
| | siRNA |
| | NRF2 siRNA (s9492) | Ambion (Austin, TX, USA) | | Control siRNA (negative control number 1) | | Lipofectamine® RNAiMAX Reagent (RNAiMAX) | Invitrogen™ (Carlsbad, CA, USA) |
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