Research Article
Formulated Chinese Medicine Shaoyao Gancao Tang Reduces Tau Aggregation and Exerts Neuroprotection through Anti-Oxidation and Anti-Inflammation
Figure 3
The effects of Bai-Shao, Gan-Cao, and SG-Tang on tau misfolding and ROS production in Tet-on ΔK280 tauRD-DsRed 293 cells. (a) Experiment flow chart. ΔK280 tauRD-DsRed 293 cells were pretreated with the tested formulas or Congo red (Congo, as a positive control) for 8 h before misfolded tau induction by doxycycline (Dox, 1 μg/ml) for three days. (b) DsRed fluorescence analysis with Congo red (5~20 μM) or the Chinese medicine formulas Bai-Shao, Gan-Cao, and SG-Tang (50~200 μg/ml) treatment (). The relative DsRed fluorescence of untreated cells is normalized (100%). , , and , treated vs. untreated cells. (c) Representative microscopy images (upper row: merged DsRed and Hoechst 33342 signals; low row: DsRed signal alone) of ΔK280 tauRD-DsRed 293 cells untreated or treated with Congo red (20 μM) or SG-Tang (200 μg/ml). (d) ROS assay of ΔK280 tauRD-DsRed 293 cells untreated or treated with Congo red (10 μM) or the tested formulas Bai-Shao (B-S), Gan-Cao (G-C), and SG-Tang (SG-T) (100 μg/ml) (). The relative ROS of untreated cells was normalized as 100%. and , treated vs. untreated cells.
| (a) |
| (b) |
| (c) |
| (d) |