Research Article
Formulated Chinese Medicine Shaoyao Gancao Tang Reduces Tau Aggregation and Exerts Neuroprotection through Anti-Oxidation and Anti-Inflammation
Figure 4
The effects of Bai-Shao, Gan-Cao and SG-Tang on neurite outgrowth in Tet-on ΔK280 tauRD-DsRed SH-SY5Y cells. (a) Experiment flow chart. ∆K280 tauRD-DsRed SH-SY5Y cells were seeded in 24-well (3 × 104/well) plate with all trans retinoic acid (RA, 10 μM). On day 2, cells were treated with Congo red (20 μM) or SG-Tang (200 μg/ml) for 8 h, induced tauRD-DsRed expression with doxycycline (Dox, 1 μg/ml), and neurite outgrowth assayed on day 9. (b) Neurite outgrowth assay () with Congo red or SG-Tang (SG-T) treatment. To normalize, the relative neurite outgrowth of untreated cells is set as 100%. , induced vs. un-induced cells; &, treated vs. untreated cells. (c) Representative microscopy images of neuronally differentiated ΔK280 tauRD-DsRed SH-SY5Y cells uninduced (− Dox), untreated (+ Dox), and after treatment with Congo red (+ Dox/Congo red) or SG-Tang (+ Dox/SG-Tang). Neurites were stained with TUBB3 (neuronal class III β-tubulin, green) antibody. Nuclei were detected using (DAPI, blue). Upper row, merged TUBB3 and DAPI signals; lower row, images of the neurites and the body of individual cells being outlined by the same color for outgrowth quantification.
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