Thymosin β4 Prevents Oxidative Stress, Inflammation, and Fibrosis in Ethanol- and LPS-Induced Liver Injury in Mice

Figure 3

Effect of Tβ4 on EtOH- and LPS-induced activation of NFκB and proinflammatory cytokine induction. Nuclear, cytosolic, or total protein was extracted from whole liver tissue from mice of various groups to determine the protein expression of (a) nuclear NFκB, (b) cytosolic NFκB, (c) total IκB, and (d) p-IκB by Western blot analysis. Total RNA was extracted from whole liver tissue from mice of various groups to determine mRNA expression using quantitative RT-PCR of (e) TNFα, (f) IL-1β, and (g) IL-6. All values are means of triplicate experiments ± SE and were corrected for loading differences after reprobing with lamin B1 (nuclear protein) or β-actin (cytosolic or total protein) or S18 (mRNA). versus control; versus EtOH; versus EtOH + LPS.

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