Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Diaphragm Dysfunction in Rats by Reducing Apoptosis and Inflammation through ROS/MAPK and TLR4/NF-κB Signaling Pathways

Figure 4

Effects of H₂S on the cytokine levels and the TLR4/NF-κB signaling pathway in diaphragmatic muscle tissues of septic rats. (a–f) The expression levels of MCP-1, TNF-α, IL-1β, IL-6, IL-10, and IL-18 were measured. (g–l) The protein expressions of TLR4, TAK1, p-TAK1, IKKα, IKKβ, p-IKKα/β, IκBα, p-IκBα, p50, p65, p-p65, and GAPDH were analyzed by Western blotting. Data are presented as mean ± SEM of three independent experiments; and compared with the control group; and compared with the LPS group.

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