Using ROS as a Second Messenger, NADPH Oxidase 2 Mediates Macrophage Senescence via Interaction with NF-κB during Pseudomonas aeruginosa Infection

Figure 1

PAO1 infection causes macrophage senescence in a colony-dependent manner. (a) Macrophages underwent senescence indicated by positive SA-β-gal staining during the PAO1 infection. (b) Percentage of senescent cells in macrophages incubated with the indicated colony counts of PAO1 for 4 and 24 h. by ANOVA test among these groups. The percentage was calculated as the mean value of the percentage of senescent cells under 5 random fields of a high-power microscope. Three independent experiments were conducted. (c) Senescent marker, p16^ink4a, expression among the different groups. Control indicates untreated macrophages incubated under standard condition for 24 h; PAO1-L-4h indicates that the macrophages were incubated with 4 × 10⁶ colony counts of PAO1 for 4 h; PAO1-L-24h indicates that the macrophages were incubated with 4 × 10⁶ colony counts of PAO1 for 24 h; PAO1-H-4h indicates that the macrophages were incubated with 1.6 × 10⁷ colony counts of PAO1 for 4 h; PAO1-H-24h indicates that the macrophages were incubated with 1.6 × 10⁷ colony counts of PAO1 for 24 h.

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