UVA Irradiation Enhances Brusatol-Mediated Inhibition of Melanoma Growth by Downregulation of the Nrf2-Mediated Antioxidant Response

Figure 1

Low-dose UVA can modulate the expression of phase II detoxification enzymes. (a) A375 cells were plated overnight and irradiated with different doses of UVA, and cell survival was determined 24 hours after UVA irradiation. (b and c) Cells were treated with UVA in the range of 0–100 kJ/m² for 24 hours. Total cell extracts were prepared for 24 hours following UVA irradiation and subjected to Western blot using antibodies for Nrf2, HO-1, NQO1, GSTP1, IκBα, COX2, NF-κB, and actin. We received a similar result in three independent experiments.

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