UVA Irradiation Enhances Brusatol-Mediated Inhibition of Melanoma Growth by Downregulation of the Nrf2-Mediated Antioxidant Response
Cotreatment increases intracellular ROS level and inhibits A375 cell growth and proliferation. (a) A375 cells were treated with UVA and BR for 24 hours. Cell morphology was observed, and cell survival analyses were performed by an MTS assay (b). Cell cycle analysis by PI staining (d and e) and cell cycle-related genes cyclinD1, cyclinE2, CDK2, CDK4, and CDK6 were performed by qRT-PCR assay (f). (c) A375 cells were pretreated with BR for 2 hours prior to UVA exposure and were then irradiated with low-dose UVA (75 kJ/m2). ROS levels were determined by flow cytometry immediately following UVA irradiation. All data are shown as the mean ± SD; ,, and . All values are based on analysis control versus treatment.
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