UVA Irradiation Enhances Brusatol-Mediated Inhibition of Melanoma Growth by Downregulation of the Nrf2-Mediated Antioxidant Response

Figure 4

Cotreatment inhibits Nrf2 and AKT pathways and leads to apoptosis in A375 cells. (a and b) A375 cells were treated with UVA and BR for 24 hours before measuring apoptosis using Annexin V and PI in conjunction with flow cytometry. (c and d) Whole-cell lysates from A375 cells were prepared and subjected to immunoblotting using antibodies against Bcl-2 family proteins, PARP, cleaved caspase-3 (C-caspase3), caspase7, caspase8, and actin. Cells were treated with UVA, BR, or both for 24 hours or different time intervals as indicated. (e) Immunoblot analysis using antibody against Nrf2 pathway proteins, AKT and p-AKT protein from A375 cell lysates. , , and . All values are based on analysis control versus treatment.

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