UVA Irradiation Enhances Brusatol-Mediated Inhibition of Melanoma Growth by Downregulation of the Nrf2-Mediated Antioxidant Response
UVA irradiation leads to suppression of A375 Nrf2 knockout cells. (a) Western blot assay was used to analyze protein expression levels of Nrf2 in A375 cells. (b) DCFH-DA-based ROS quantification. (c) A375 Nrf2−/− irradiated with 75 kJ/m2 UVA, followed by MTS assay (d) cell cycle analysis by PI staining after 24 and 48 hours. (f) Apoptosis assay using Annexin V and PI to analyzed Nrf2−/− cells after 48 hours irradiation. (g) Colony formation was examined by staining colonies with crystal violet. Colonies with more than 50 cells were counted (). .
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