UVA Irradiation Enhances Brusatol-Mediated Inhibition of Melanoma Growth by Downregulation of the Nrf2-Mediated Antioxidant Response

Figure 6

UVA irradiation leads to suppression of A375 Nrf2 knockout cells. (a) Western blot assay was used to analyze protein expression levels of Nrf2 in A375 cells. (b) DCFH-DA-based ROS quantification. (c) A375 Nrf2^−/− irradiated with 75 kJ/m² UVA, followed by MTS assay (d) cell cycle analysis by PI staining after 24 and 48 hours. (f) Apoptosis assay using Annexin V and PI to analyzed Nrf2^−/− cells after 48 hours irradiation. (g) Colony formation was examined by staining colonies with crystal violet. Colonies with more than 50 cells were counted (). .

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