Neuroprotective Effect of Caryocar brasiliense Camb. Leaves Is Associated with Anticholinesterase and Antioxidant Properties

Figure 1

Determination of cholinesterase activity (nmol/min/μg protein) in mice brain homogenate using acetylthiocholine 1.5 mM (a) and butyrylthiocholine 1.5 mM (b) as enzyme substrates and in the presence of iso-OMPA 10 μM (an inhibitor of butyrylcholinesterase) or BW284c51 10 μM (an inhibitor of acetylcholinesterase). Control samples represent the total cholinesterase activity obtained in the absence of enzyme inhibitors. Data were obtained as a mean ± epm of 3 different samples in triplicate.

(a)

(b)