Troxerutin Protects Kidney Tissue against BDE-47-Induced Inflammatory Damage through CXCR4-TXNIP/NLRP3 Signaling

Figure 3

Troxerutin blocks the activity of SDF-1 and CXCR4 in the kidney of BDE-47-treated mice. (a) The expression of SDF-1 and CXCR4 was measured by western blot analysis. The relative density is normalized to β-actin. and , respectively, means versus the BDE-47 group (). (b) shows renal CXCR4 immunofluorescence (Leica 4000, ×40). Nuclei were stained with DAPI (blue fluorescence). Scale bar, 50 μm. White arrow indicates positive signal which was placed in the upper right corner (red). (c) CXCR4 siRNA decreased ACR in BDE-47-treated mice. and versus the adjacent groups (). All CR4 si means CXCR4 siRNA, and Scr means scramble. (d) CXCR4 siRNA suppressed the activity of kidney NLRP3 inflammasome in BDE-47-treated mice. , , and versus the adjacent groups (). (e) CXCR4 siRNA decreased the activity of NF-κB, iNOS, and COX-2. , , and versus the adjacent groups (). The relative density is normalized to β-actin or H3 ().

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