Amyloid Peptide β1-42 Induces Integrin αIIbβ3 Activation, Platelet Adhesion, and Thrombus Formation in a NADPH Oxidase-Dependent Manner

Figure 1

β amyloid peptides in static conditions support platelet adhesion. Human platelet suspensions were plated on glass coverslips coated with 10 μM scrambled Aβ1-42 (scAβ42), Aβ1-42, Aβ1-40, and Aβ25-35. The adhered platelets after 30 minutes shown in (a) were fixed, permeabilized, and stained with TRITC-conjugated phalloidin and are representative images at 10x magnification. The quantification of the adhered platelets evaluated as the mean number of platelets per optical field is shown in (b). Statistical significance for 4 independent experiments was analysed by one-way ANOVA with Bonferroni posttest (), with bars representing standard error of the mean (SEM).

(a)

(b)