Amyloid Peptide β1-42 Induces Integrin αIIbβ3 Activation, Platelet Adhesion, and Thrombus Formation in a NADPH Oxidase-Dependent Manner

Figure 3

Effect of NOX inhibitor VAS2870 on platelet adhesion to Aβ1-42. Human platelet suspension was preincubated with NOX inhibitor VAS2870 (10 μM) for 30 mins then plated on glass coverslips coated with 10 μM Aβ1-42 and 5 mg/ml BSA in PBS. The numbers of the adhered platelets fixed, permeabilized, and stained with TRITC-conjugated phalloidin are shown in (a), and representative images at 10x magnification are displayed. The quantification of the adhered platelets evaluated as the mean number of the adhered platelets per optical field is shown (b). Statistical significance for 4 independent experiments was analysed by one-way ANOVA with Bonferroni posttest (), with bars representing standard error of the mean (SEM).

(a)

(b)