Research Article

Malaysian Tualang Honey Inhibits Hydrogen Peroxide-Induced Endothelial Hyperpermeability

Figure 4

Localization of β-catenin and caveolin-1 in HUVECs pretreated with TH and induced with H2O2. Cellular localization of β-catenin (red) and Cav-1 (green) is shown merged with nucleus (blue) stained with DAPI. Merged images were generated with Olympus BX-51 Cell-F imaging software, whereby a third pseudocolour channel (orange) was used to show colocalization of red and green pixels. Representative images were taken from one of three independent experiments. Basal = untreated; TH = Tualang honey; TR = Trolox.