Excessive Neutrophil Extracellular Trap Formation Aggravates Acute Myocardial Infarction Injury in Apolipoprotein E Deficiency Mice via the ROS-Dependent Pathway

Figure 3

ApoE deficiency promotes neutrophil extracellular trap formation. (a) Representative immunofluorescent staining of anti-cit-H3 (anticitrullinated histone H3, green), Ly6G (red), and DAPI (blue) within the hearts of WT and ApoE^-/- mice 1 day after ligation and the percentage of cit-H3-positive NETs of the whole image. Each group involves 6 mice. Scale bar: 50 μm. (b) Representative immunofluorescent staining of anti-cit-H3 (red), SYTOX Green (green), and DAPI (blue) within the hearts of WT and ApoE^-/- mice 1 day after ligation and the percentage of cit-H3-positive NETs of the whole image. Each group involves 6 mice. Scale bar: 200 μm. (c) Representative dihydroethidium staining and quantitation of the mean gray value (integrated ) of the hearts of WT mice, ApoE^-/- mice, and ApoE^-/- mice injected with APOE3 1 day after ligation. Scale bar: 50 μm. Each group involves 6 mice. Data are shown as . Statistical tests include the two-tailed Student’s -test (two groups), one-way analysis of variance followed by Dunnett’s multiple comparison test (b), and Kruskal-Wallis test followed by Dunn’s multiple comparison test (c). and .

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