Excessive Neutrophil Extracellular Trap Formation Aggravates Acute Myocardial Infarction Injury in Apolipoprotein E Deficiency Mice via the ROS-Dependent Pathway

Figure 6

The role of excessive NET formation in myocardial infarction injury in ApoE^-/- mice. (a, b) Representative TTC staining and the percentage of infarct size of WT or ApoE^-/- mice with PBS, apocynin, or Cl-amidine injection 1 day after ligation. Each group involves 6 mice. (c) Serum CK-MB level of WT or ApoE^-/- mice with PBS, apocynin, or Cl-amidine injection 1 day after ligation. Each group involves 8 mice. (d) Representative immunohistochemistry staining of cit-H3 (citrullinated histone H3, red), SYTOX Green (green), and DAPI (blue) within the hearts of WT or ApoE^-/- mice with PBS, apocynin, or Cl-amidine injection 1 day after ligation. And the quantitation of the percentage of the cit-H3-positive area of the whole image. Scale bar: 100 μm. Each group involves 6 mice. Columns filled with black: WT mice. Columns filled with white: ApoE^-/- mice. Data are shown as . Statistical tests include the two-tailed Student’s -test (two groups) and one-way analysis of variance followed by Dunnett’s multiple comparison test (more than two groups). and .

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