N-Acetyl Cysteine as a Novel Polymethyl Methacrylate Resin Component: Protection against Cell Apoptosis and Genotoxicity

Figure 1

PMMA-induced ROS accumulation triggers cell death and its prevention by NAC incorporation. (a) Schematic representation of the coculture of hDPCs with the experimental PMMA resin specimens in the Transwell system. Experimental PMMA resin specimens were placed in the inserts (upper compartments), and hDPCs were seeded in the wells (lower compartments). (b) Viability of hDPCs as examined by the CCK-8 assay. (c) Visualization of hDPC viability observed by phase contrast microscopy after a 24 h exposure period. (d) Intracellular ROS level evaluated by flow cytometry after a 1 h exposure period. Data represent (). , , and vs. untreated cells (control group); ^#, ^##, and ^### vs. PMMA-treated cells. Data were analyzed using one-way analysis of variance (ANOVA) or two-way ANOVA and post hoc Tukey’s test.

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