N-Acetyl Cysteine as a Novel Polymethyl Methacrylate Resin Component: Protection against Cell Apoptosis and Genotoxicity

Figure 2

Induction of apoptosis and necrosis in PMMA-treated hDPCs. After a 24 h exposure period, cells were stained with annexin V-FITC/propidium iodide (PI) and analyzed by flow cytometry. (a) Percentages of viable cells (unstained, B3) and cells in apoptosis (annexin, B4), late apoptosis (annexin and PI, B2), and necrosis (PI, B1) of one typical experiment are denoted in the quadrants of each density blot. (b) Bar graphs represent flow cytometry data. Data represent (). , , and vs. untreated cells (control group); ^#, ^##, and ^### vs. PMMA-treated cells. Data were analyzed using one-way analysis of variance (ANOVA) and post hoc Tukey’s test.

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