Immunohistochemical Study of Antioxidant Enzymes Regulated by Nrf2 in the Models of Epileptic Seizures (KA and PTZ)

Figure 1

Representative micrographs of the hippocampal fields of rats treated with KA. Area in CA1 in the control hippocampus (a–d), 1 h group (e–h), 6 h group (i–l), 12 h group (m–p), 24 h group (q–t), and 48 h group (u–x). The sections stained with cresyl violet, showing neuronal cells with karyolysis (^), pyknotic nuclei, interstitial edema, and cellular necrosis () (i, m, q, and u). A GPx-, SOD-, and CAT-positive staining of CA1 and CA3 neurons can be observed. The hippocampal tissues increased GPx, SOD, and CAT immunoreactivity mainly in neurons and astrocyte with nuclei strongly stained in the experimental groups (↑). Preparations were stained for cresyl violet (a, e, i, m, q, and u) and immunostained for GPx (b, f, j, n, r, and v), SOD (c, g, k, o, s, and w), and CAT (d, h, l, p, t, and x) were observed. Magnification: 1000x. Scale bar: 20 μ.