Gamma Radiation-Induced Disruption of Cellular Junctions in HUVECs Is Mediated through Affecting MAPK/NF-κB Inflammatory Pathways

Figure 2

Cellular junction examination in HUVECs after exposure to gamma irradiation. Immunofluorescence examination indicates the damage of the cellular barrier 24 h after irradiation with 5 Gy (the impaired and disconnected junctions between two adjoined cells pointed by arrows in the lower panels in (a)) (×400). Scale bar: 50 μm. While the gene expressions of junction-related molecules such as ZO-1, occludin, VE-cadherin, and connexin 40 do not change significantly (b), there are obvious reductions of protein expressions of ZO-1, occludin, VE-cadherin, and connexin 40 (c).

(a)

(b)

(c)