Gamma Radiation-Induced Disruption of Cellular Junctions in HUVECs Is Mediated through Affecting MAPK/NF-κB Inflammatory Pathways

Figure 5

Gamma irradiation activates MAPK/NF-κB pathways. (a) The activation of MAPK pathway molecules p-p38, p38, p53, p21, and p27 in HUVECs after exposure to gamma irradiation was measured by western blot. (b) Protein levels of p-p65 and p65 were examined by western blot. (c) NF-κB DNA-binding activity by TransAM™ NF-κB Transcription Factor Assay kit. The absorbance at 450 nm was read on a microplate reader. Values were expressed as One-way analysis of variance (ANOVA) was used to determine statistical significance between groups followed by Tukey’s post hoc test. A value of was taken as statistical significance.

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