5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy

Figure 10

Epigenetic alternations during chondrogenesis in control and experimental conditions. Prior to experiments, cells were pretreated with AZA/RES for 24 hours, then the experimental medium was replaced by osteogenic differentiation medium. Cells were cultured in that medium for five days and after were subjected to further analysis. Epigenetic alternations in cells were investigated with flow cytometry. Representative graphs show results of 5-mC and H3 accumulation in cells (a). Obtained results revealed decreased levels of 5-mC in EMS EXP (b). Increased H3 amount was noted in the EMS group (c). Expression of TET-2 (d) and TET-3 (e) was analyzed with RT-PCR. Results expressed as . Statistical significance indicated as an asterisk () when comparing the result to ASC_EMS and as a number sign (#) when comparing to ASC_CTRL. ^#, .

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