5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy

Figure 11

Evaluation of anti-inflammatory properties of prechondroblastic cells. The effect of AZA/RES on the activation status of RAW 264.7 macrophages. RAW 264.7 were seeded at a density of cells/mL, and after 18 hours, not-adherent cells were removed. Next, LPS was added to the culture media at a concentration of 1 μg/mL, and the experiment was continued for another 24 h. At the same time, ASCs after the fifth day of differentiation in the amount of were added to culture wells. After 24 hours of coculture, media were collected for analysis of the macrophages’ secretory activity, and the cells were lysed by adding TRI Reagent. Arginase-1 (a), iNOS (b), TNF-α (c), and IL-6 (d) gene expression was evaluated in macrophages with RT-PCR. Results expressed as . Statistical significance indicated as an asterisk () when comparing the result to ASC_EMS and as a number sign (#) when comparing to ASC_CTRL. ^##, ^### and .

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