Research Article
5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy
Figure 7
Evaluation of mitochondrial net types. Prior to experiments, cells were pretreated with AZA/RES for 24 hours, then the experimental medium was replaced by osteogenic differentiation medium. Cells were cultured in that medium for five days and after were subjected to further analysis. Mitochondrial net visualization was performed in the Mitochondrial Network Analysis (MiNA) toolset using the photographs from MitoRed staining (a). Obtained images were further quantified in the same software. The mean number of both individuals (b) and networks (c) was substantially greater in EMS than in EMSEXP. The number of mean branches per network was significantly increased in EMXEXP (d). The ratio of rod to branch was diminished in EMS cells; however, after treatment with AZA/RES, increased value of that parameter was noted (e). Results expressed as . Statistical significance indicated as an asterisk () when comparing the result to ASCEMS and as a number sign (#) when comparing to ASCCTRL. # and ; ; ### and .
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