Research Article
5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy
Table 2
Sequences of primers used for RT-PCR.
| | Gene name | Forward and reverse primer sequence (5-3) | Size of amplicon (bp) | Accession no. |
| | Vimentin | F: GCAGGATTTCTCTGCCTCTT | 203 | XM_014846038.1 | | R: TATTGCTGCACCAAGTGTGT | | Decorin | F: GATGCAGCTAGCCTGAGAGG | 248 | XM 014841263.1 | | R: GTGTTGTATCCAGGTGGGCA | | COMP | F: AGTGTCGCAAGGATAACTGCGTGA | 238 | NM_001081856.1 | | R: TCCTGATCTGTGTCCTTCTGGTCA | | SOX-9 | F: GAACGCCTTCATGGTGTGGG | 225 | XM_014736619.1 | | R: TTCTTCACCGACTTCCTCCG | | p53 | F: TACTCCCCTGCCCTCAACAA | 252 | U37120.1 | | R: AGGAATCAGGGCCTTGAGGA | | p21 | F: GAAGAGAAACCCCCAGCTCC | 241 | XM_014853747.1 | | R: TGACTGCATCAAACCCCACA | | Caspase-3 | F: GGCAGACTTCCTGTATGCGT | 167 | XM_023630401.1 | | R: CCATGGCTACCTTGCGGTTA | | Bcl-2 | F: TTCTTTGAGTTCGGTGGGGT | 164 | XM_014843802.1 | | R: GGGCCGTACAGTTCCACAA | | BAX | F: TTCCGACGGCAACTTCAACT | 150 | XM 005607505.1 | | R: GGTGACCCAAAGTCGGAGAG | | CHOP | F: AGCCAAAATCAGAGCCGGAA | 272 | XM 014844003.1 | | R: GGGGTCAAGAGTGGTGAAGG | | PERK | F: GTGACTGCAATGGACCAGGA | 283 | XM 014852775.1 | | R: TCACGTGCTCACGAGGATATT | | eIF2α | F: AGTCTTCAGGCATTGGCTCC | 489 | XM_001488848.6 | | R: CCGAGTGGGACATGTATCGG | | Beclin-3 | F: GATGCGTTATGCCCAGATGC | 233 | XM 014833759.1 | | R: AACGGCAGCTCCTCTGAAAT | | LAMP-2 | F: GCACCCCTGGGAAGTTCTTA | 139 | XM 014733098.1 | | R: ATCCAGCGAACACTCTTGGG | | p62 (SQSTM) | F: CATCGGAGGATCCCAGTGTG | 207 | XM_005599173.3 | | R: CCGGTTTGTTAGGGTCGGAA | | LC3 | F: TTACTGCTTTGCTCTGCCAC | 213 | XM 005608485.2 | | R: AGCTGCTTCTCCCCCTTGT | | mTOR | F: GGGCAGCATTAGAGACGGTG | 221 | XM 005607537.2 | | R: ATGGTTGATTCGGTGTCGCA | | TET-2 | F: ATCCTGATCCTGGTGTGGGA | 143 | XM_023636796.1 | | R: CCTTGACAGGCACAGGTTCT | | TET-3 | F: CAGCCTGCATGGACTTCTGT | 188 | XM_023618871.1 | | R: GTTCTCCTCACTGCCGAACT | | FIS | F: GGTGCGAAGCAAGTACAACG | 118 | XM 001504462.4 | | R: GTTGCCCACAGCCAGATAGA | | PINK | F: GCACAATGAGCCAGGAGCTA | 298 | XM 014737247.1 | | R: GGGGTATTCACGCGAAGGTA-0 | | PARKIN | F: TCCCAGTGGAGGTCGATTCT | 218 | XM 014858374.1 | | R: CCCTCCAGGTGTGTTCGTTT | | MFN1 | F: AAGTGGCATTTTTCGGCAGG | 217 | XM 001495170.5 | | R: TCCATATGAAGGGCATGGGC | | Arginase-1 | F: CCAGAAGAATGGAAGAGTCAGTGT | 252 | NM_007482.3 | | R: GCAGATATGCAGGGAGTCACC | | iNOS | F: GACAAGCTGCATGTGACATC | 325 | XM_006532446.3 | | R: GCTGGTAGGTTCCTGTTGTT | | TNF-α | F: ACAGAAAGCATGATCCGCGA | 295 | NM_013693.3 | | R: CTTGGTGGTTTGCTACGACG | | IL-6 | F: GAGGATACCACTCCCAACAGACC | 146 | NM_001314054.1 | | R: AAGTGCATCATCGTTGTTCATACA | | GAPDH | F: GATGCCCCAATGTTTGTGA | 250 | NM 001163856.1 | | R: AAGCAGGGATGATGTTCTGG | | Beta actin | CATACGCCTGCAGAGTTAAGCA | 73 | NM_009735.3 | | GATCACATGTCTCGATCCCAGTAG |
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Sequences: amplicon length and access numbers of the primer sets. COMP: cartilage oligomeric matrix protein; Sox-9: transcription factor SOX-9; p21: cyclin-dependent kinase inhibitor 1A; p53: tumor suppressor p53; BCL-2: B-cell lymphoma 2; BAX: BCL-2-associated X protein; CHOP: DNA damage inducible transcript 3; PERK: PRKR-like endoplasmic reticulum kinase; eIFα: eukaryotic initiation factor 2; Beclin-3: autophagy related (BECN1); LAMP2: lysosomal-associated membrane protein 2; p62: Sequestosome-1; LC3: microtubule associated protein 1 light chain 3 beta (MAP1LC3B); mTOR: mammalian target of rapamycin; TET 2: Tet methylcytosine dioxygenase 2; TET 3: Tet methylcytosine dioxygenase PINK: PTEN-induced putative kinase 1 (PINK1); PARKIN: parkin RBR E3 ubiquitin protein ligase (PARK2); FIS: mitochondrial fission 1 molecule; MFN1: mitofusin 1; iNOS: inducible nitric oxide synthase; TNF-α: tumor necrosis factor alpha; IL-6: interleukin 6 ; GADPH: glyceraldehyde-3-phosphate dehydrogenase.
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