Flavonoids from Apios americana Medikus Leaves Protect RAW264.7 Cells against Inflammation via Inhibition of MAPKs, Akt-mTOR Pathways, and Nfr2 Activation

Figure 5

ALE treatment altered expressions of proteins associated with inflammation and oxidative stress in RAW264.7 cells. (a) Western blot bands of iNOS, COX2, NF-κB, and Nrf2-Keap1 (). (b) Western blot bands of MAPKs. (c–j) Relative expressions of iNOS, COX2, NF-κB, Keap1, Nrf2, p-JNK/JNK, p-ERK/ERK, and p-p38/p38. The images were quantified by the ImageJ software, and the intensity of bands was corrected with β-actin (); vertical lines in the histogram represent SDs of three replicates. Significance analysis was carried out according to the one-way ANOVA test, and different letters mean statistically significant differences among the groups (A, B, C, D, and E were labeled from large to small and the same letters in columns mean statistical insignificance; otherwise, they mean statistical significance ()).

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