Flavonoids from Apios americana Medikus Leaves Protect RAW264.7 Cells against Inflammation via Inhibition of MAPKs, Akt-mTOR Pathways, and Nfr2 Activation

Figure 7

Effect of ALE on expression levels of HMGB1, FoxO1, and Akt-mTOR pathways in RAW264.7 cells. (a) Western blot bands of HMGB1, p-FOXO1, FOXO1, p-Akt, Akt, p-mTOR, mToR, and β-actin (). (b–e) Relative expression levels of HMGB1, p-FOXO1/FOXO1, p-Akt/Akt, and p-mTOR/mToR. The images were quantified by the ImageJ software, and the intensity of bands was corrected with β-actin (); vertical lines in the histogram represent SDs of three replicates. (f) The schematic of the mechanism of impact of ALE on LPS-induced RAW264.7 cells. Significance analysis was carried out according to the one-way ANOVA test, and different letters mean statistically significant differences among the groups (A, B, C, D, and E were labeled from large to small and the same letters in columns mean statistical insignificance; otherwise, they mean statistical significance ()).

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