Antimycin A-Induced Mitochondrial Damage Causes Human RPE Cell Death despite Activation of Autophagy

Figure 1

Aa treatment was toxic to human RPE cells. Aa caused a dose- and time-dependent decrease in cellular viability (a, b) and a corresponding increase in cellular toxicity (c) in ARPE-19 cells. In hRPE cells (9 donors), treatment with 20 μM Aa also reduced MTT (d) levels and increased LDH release (e). Experiments were performed at least three times with 4-6 parallels in each group per experiment. Results are presented as . ns: not statistically significant; , , and compared to vehicle control of the same time point; , , and for MTT samples measured 48 or 72 hours after Aa stimulation and compared to the 24-hour time point, Mann-Whitney test.

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