Antimycin A-Induced Mitochondrial Damage Causes Human RPE Cell Death despite Activation of Autophagy

Figure 2

Evidence of apoptosis after Aa exposure. Treatment with Aa led to an increase in the number of TUNEL-positive cells in both ARPE-19 (a) (scale bar: 100 μm; green: TUNEL; blue: DAPI) and hRPE cells (b) (scale bar: 100 μm; green: TUNEL; blue: DAPI; red: autofluorescence of pigment in hRPE cells; 3 different donors). Concurrently, there was an increase in caspase 3 activity in ARPE-19 cells (c). Results are a combination of three independent experiments with 2 (a, b) or 4 (c) parallels per group. Results are presented as . Microscopy images shown are representative. and , Mann-Whitney test.

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