Antimycin A-Induced Mitochondrial Damage Causes Human RPE Cell Death despite Activation of Autophagy

Figure 7

Inhibition of autophagy increased cell death in antimycin A- (Aa-) treated ARPE-19 cells. Inhibition of autophagy in Aa-treated cells using 50 nM bafilomycin A1 (BafA) or 30 μM chloroquine (CQ) decreased cellular viability measured by the MTT assay and increased the release of LDH into the culture medium. This increased cell toxicity was greater after Aa treatment than in the absence of mitochondrial damage. Results are combined from three independent experiments with six parallels per group. Results are presented as . ns: not statistically significant; and , Mann-Whitney test.

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