4-Hydroxychalcone Induces Cell Death via Oxidative Stress in MYCN-Amplified Human Neuroblastoma Cells

Figure 5

4HC-induced NB cell death is prevented by inhibition of oxidative stress. (a, b) Cell viability measured by MTT assay and (c) representative photomicrographs of (a, c) SK-N-BE (2) and (b, c) IMR-32 cells pretreated with NAC before being cultured with or without 25 μM 4H for SK-N-BE (2) and 10 μM 4H for IMR-32 C for 24 h. . Cell viability measured by MTT assay on (d) SK-N-BE (2) and (E) IMR-32 cells pretreated with IM-54 before being cultured with or without 25 μM 4HC for SK-N-BE (2) and 10 μM 4HC for IMR-32 for 24 h. All data are ; independent experiments. and versus control or as indicated; ANOVA with Fisher’s LSD test).

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