Age-Dependent Oxidative Stress Elevates Arginase 1 and Uncoupled Nitric Oxide Synthesis in Skeletal Muscle of Aged Mice

<table class="figure-group"><tr class="fig-image" id="a"><td><object data="https://static.hindawi.com/articles/omcl/volume-2019/1704650/figures/1704650.fig.003a.svgz" name="1704650.fig.003a" type="image/svg+xml"></object></td></tr><tr class="fig-caption"><td><b>(a) </b></td></tr><tr class="fig-image" id="b"><td><object data="https://static.hindawi.com/articles/omcl/volume-2019/1704650/figures/1704650.fig.003b.svgz" name="1704650.fig.003b" type="image/svg+xml"></object></td></tr><tr class="fig-caption"><td><b>(b) </b>3-NT</td></tr><tr class="fig-image" id="c"><td><object data="https://static.hindawi.com/articles/omcl/volume-2019/1704650/figures/1704650.fig.003c.svgz" name="1704650.fig.003c" type="image/svg+xml"></object></td></tr><tr class="fig-caption"><td><b>(c) </b>eNOS-dimer/monomer ratio</td></tr></table>

<div>Elevated level of peroxynitrite (ONOO<sup>-</sup>) formation and uncoupling of eNOS in aged muscle. (a) Representative western blots of protein extracted from young and old muscle samples for 3-NT and eNOS uncoupling. Densitometry quantification of (b) 3-NT and (c) eNOS uncoupling. Values are normalized to the expression levels of the housekeeping gene GAPDH. 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Oxidative Medicine and Cellular Longevity

fig3

Figure 3

Figure 3: Age-Dependent Oxidative Stress Elevates Arginase 1 and Uncoupled Nitric Oxide Synthesis in Skeletal Muscle of Aged Mice 