Age-Dependent Oxidative Stress Elevates Arginase 1 and Uncoupled Nitric Oxide Synthesis in Skeletal Muscle of Aged Mice

Figure 4

Effect of oxidative stress on arginase activity on myoblasts. C2C12 cells were incubated in DMEM (2% FBS, 50 mM L-arginine) with and without hydrogen peroxide (50 μM) for 48 h. Arginase activity in cell lysate was determined by the arginase activity assay (, ).