Age-Dependent Oxidative Stress Elevates Arginase 1 and Uncoupled Nitric Oxide Synthesis in Skeletal Muscle of Aged Mice

Figure 5

Arginase inhibitor prevents C2C12 cells from oxidative stress damage. (a) C2C12 cells were treated with H₂O₂ (50 and 100 μM) in the presence or absence of ABH (100 μM) for 24 h. MTS analysis was performed after 24 h following treatment. (b) Representative western blots for total PARP and cleaved PARP on C2C12 cells. (c) Densitometry ratio of total PARP and cleaved PARP. Values are normalized to the expression levels of the housekeeping gene GAPDH. Data were analyzed by one-way ANOVA followed by the Bonferroni post hoc test (, ).

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