Blockade of Cyclophilin D Attenuates Oxidative Stress-Induced Cell Death in Human Dental Pulp Cells

Figure 2

NAC attenuated H₂O₂-induced cell death and mitochondrial dysfunction in the HDPCs. (a) Cell viability determined by MTT reduction in the HDPCs in the presence of H₂O₂ with or without NAC. (b) TUNEL staining and (c) assay after NAC treatment. (d) Representative immunoreactive bands for Bax in the HDPCs with (+) or without (-) NAC treatment in the presence of H₂O₂ (+) or culture medium (-). Quantification of immunoreactive bands for Bax (e) relative to β-actin. Representative images showing MitoSOX staining (f) and quantification (g) in the indicated groups. Representative images showing TMRM staining (h) and quantification (i) in the indicated groups. Representative images showing Fluo-4-AM staining (j) and quantification (k) in the indicated groups. ATP (l) in the indicated groups. (m) Densitometry immunoreactive bands for CypD in the HDPCs with (+) or without (-) NAC treatment in the presence of H₂O₂ (+) or culture medium (-). (n) Quantification of immunoreactive bands for CypD relative to β-actin. HDPCs were treated for 24 h with (+) or without (−) NAC (2.5 mM) in the presence of H₂O₂ (250 μM) (+) or culture medium (−). Data represent the mean of three independent experiments.

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