Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 2

Research Article

Blockade of Cyclophilin D Attenuates Oxidative Stress-Induced Cell Death in Human Dental Pulp Cells

Figure 2

NAC attenuated H2O2-induced cell death and mitochondrial dysfunction in the HDPCs. (a) Cell viability determined by MTT reduction in the HDPCs in the presence of H2O2 with or without NAC. (b) TUNEL staining and (c) assay after NAC treatment. (d) Representative immunoreactive bands for Bax in the HDPCs with (+) or without (-) NAC treatment in the presence of H2O2 (+) or culture medium (-). Quantification of immunoreactive bands for Bax (e) relative to β-actin. Representative images showing MitoSOX staining (f) and quantification (g) in the indicated groups. Representative images showing TMRM staining (h) and quantification (i) in the indicated groups. Representative images showing Fluo-4-AM staining (j) and quantification (k) in the indicated groups. ATP (l) in the indicated groups. (m) Densitometry immunoreactive bands for CypD in the HDPCs with (+) or without (-) NAC treatment in the presence of H2O2 (+) or culture medium (-). (n) Quantification of immunoreactive bands for CypD relative to β-actin. HDPCs were treated for 24 h with (+) or without (−) NAC (2.5 mM) in the presence of H2O2 (250 μM) (+) or culture medium (−). Data represent the mean of three independent experiments.
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