Blockade of Cyclophilin D Attenuates Oxidative Stress-Induced Cell Death in Human Dental Pulp Cells

Figure 3

CsA attenuated H₂O₂-induced cell death and mitochondrial dysfunction in the HDPCs. (a) Cell viability determined by MTT reduction in the HDPCs in the presence of H₂O₂ with or without CsA. (b) TUNEL staining and (c) assay after CsA treatment. (d) Representative immunoreactive bands for Bax and CypD in the HDPCs with (+) or without (-) CsA treatment in the presence of H₂O₂ (+) or culture medium (-). Quantification of immunoreactive bands for Bax (e) and CypD (f) relative to β-actin. Representative images showing MitoSOX staining (g) and quantification (h) in the indicated groups. Representative images showing TMRM staining (i) and quantification (j) in the indicated groups. ATP (m) in the indicated groups. Representative images showing Fluo-4-AM staining (k) and quantification (l) in the indicated groups. HDPCs were treated for 24 h with (+) or without (−) CsA (2 μM) in the presence of H₂O₂ (250 μM) (+) or culture medium (−). Data represent the mean of three independent experiments.

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