Blockade of Cyclophilin D Attenuates Oxidative Stress-Induced Cell Death in Human Dental Pulp Cells

Figure 4

CypD siRNA attenuated H₂O₂-induced cell death and mitochondrial dysfunction in the HDPCs. (a) Representative immunoreactive bands for CypD in the HDPCs with CypD siRNA (+) or NC (-) treatment at a final concentration of 50 nM for 48 h. Quantification of immunoreactive bands for CypD (b) relative to β-actin. (c) Cell viability determined by MTT reduction in the HDPCs in the presence of H₂O₂ with or without siRNA-PPIF. (d) TUNEL staining and (e) assay after siRNA-PPIF treatment. (f) Representative immunoreactive bands for Bax, Bcl-2, and CypD in the HDPCs with (+) or without (-) siRNA-PPIF treatment in the presence of H₂O₂ (+) or culture medium (-). Quantification of immunoreactive bands for Bax (g), Bcl-2 (h), and CypD (i) relative to β-actin. Representative images showing MitoSOX staining (j) and quantification (k) in the indicated groups. Representative images showing TMRM staining (l) and quantification (m) in the indicated groups. ATP (n) in the indicated groups. Representative images showing Fluo-4-AM staining (o) and quantification (p) in the indicated groups. HDPCs were treated for 24 h with H₂O₂ (250 μM) (+) or culture medium (−) in the CypD siRNA group (+) or the NC group (−). Data represent the mean of three independent experiments.

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