A Quantitative Method to Measure Low Levels of ROS in Nonphagocytic Cells by Using a Chemiluminescent Imaging System

Table 2

Reagents and stock preparation for the optimized chemiluminescence buffer.

Component	Stock concentration	Storage	Working concentration
Luminol	50 mM, dissolved in 0.1 M NaOH or DMSO	Stored in small aliquots at -20°C	1 mM (diluted in HBSS)
4IPBA	90 mM, dissolved in DMSO	Stored in small aliquots at -20°C	3.6 mM (diluted in HBSS)
HRP	200 units/ml, dissolved in PBS	Stored in small aliquots at -20°C	4 units/ml (diluted in HBSS)
NAC	500 mM, dissolved in distilled water	Stored in small aliquots at -20°C	200 mM (diluted in HBSS)
DPI	1 mM, dissolved in DMSO	Stored in small aliquots at -20°C	200 μM (diluted in HBSS)
SOD	1 mg/ml, dissolved in PBS	Stored in small aliquots at -20°C	1 mg/ml
CAT	30 KU/ml, dissolved in PBS	Stored in small aliquots at -20°C	30 KU/ml