Polyphyllin VII Promotes Apoptosis and Autophagic Cell Death via ROS-Inhibited AKT Activity, and Sensitizes Glioma Cells to Temozolomide

Figure 7

PP7 reduces TMZ resistance in U251 and U87-MG cells by suppressing the expression of MGMT. (a) Quantified mRNA expression of MGMT after TMZ treatment in U251 cells (). (b) PP7 suppresses the expression of MGMT induced by TMZ treatment in U251 cells (). (c) Quantified mRNA expression of MGMT after TMZ treatment in U87-MG cells (). (d) PP7 suppresses the expression of MGMT induced by TMZ treatment in U87-MG cells (). (e, f) Western blots and corresponding quantification analysis show that TMZ activates the phosphorylation of AKT, which could be inhibited by PP7 in U251 cells (). (g, h) Western blots and corresponding quantification show that TMZ activates the phosphorylation of AKT, which could be inhibited by PP7 in U87-MG cells (). Solvent controls are represented as 0 μM. stands for the repetition of experiments. , , .

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