Potential of Mitochondria-Targeted Antioxidants to Prevent Oxidative Stress in Pancreatic β-cells

Figure 8

Isolated pancreatic islets: (a) prooxidant effects or antioxidant effects of matrix-targeted antioxidants SkQ1, S1QEL, and S3QEL on natural 4 hr ROS accumulation estimated by MitoB; (b) viability after 4 hr. Prooxidant activity (>100%) or antioxidant (<100%) activity is related to ROS accumulated after 4 hr in isolated pancreatic islets. Normalization, i.e., 100%, was taken to be the measurement with no agent added (a) or as % of live cells (b). Medium either contained 5 mmol·l^-1 glucose (vs. Glc5), nonstimulating with regard to insulin secretion or 25 mmol·l^-1 glucose (vs. Glc25), stimulating insulin secretion. Where indicated, 1 nmol·l^-1 SkQ1, 10 μmol·l^-1 S1QEL, or S3QEL were added at the beginning of 4 hr incubations. The error bars represent the standard deviation of 3 measurement. Student’s -test gave statistical significance where indicated as follows: ; .

(a)

(b)